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Pappula, Nagaraju
- A Validated Reverse Phase HPLC Method for the Simultaneous Estimation of Ritonavir and Lopinavir in Pharmaceutical Dosage Forms
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Authors
Affiliations
1 Hindu College of Pharmacy, Guntur-522002, Andhra Pradesh, IN
2 College of Pharmacy, Al-Jabal Al-Gharbi University, Al-Zawia, LY
3 Centre for Pharmacy, JNT University, Hyderabad, Andhra Pradesh, IN
1 Hindu College of Pharmacy, Guntur-522002, Andhra Pradesh, IN
2 College of Pharmacy, Al-Jabal Al-Gharbi University, Al-Zawia, LY
3 Centre for Pharmacy, JNT University, Hyderabad, Andhra Pradesh, IN
Source
Asian Journal of Research in Chemistry, Vol 3, No 3 (2010), Pagination: 805-807Abstract
A simple, sensitive and precise reverse phase high performance liquid chromatographic method has been developed for the simultaneous estimation of Ritonavir and Lopinavir in pharmaceutical dosage forms. The mobile phase consisted of Acetonitrile: buffer (0.05 M Potassium dihydrogen orthophosphate, PH-3) in the ratio of 50:50 v/v delivered at a flow rate of 2.0 ml/min and wavelength of detection at 215 nm. The retention times of Ritonavir and Lopinavir were 10.253 min and 12.490 min respectively. The developed method was validated according to ICH guidelines. The proposed method can be used for determination of these drugs in combined dosage forms.Keywords
Ritonavir, Lopinavir, RP-HPLC.
- Development and Validation of Stability Indicating Uhplc Method for Simultaneous Estimation of Ombitasvir, Paritaprevir and Ritonavir in Pharmaceutical Dosage Forms
Abstract Views :86 |
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Authors
Affiliations
1 Professor, Department of Pharmaceutical Analysis, Hindu College of Pharmacy, Guntur - 522002, A.P, IN
2 Research Scholar, Acharya Nagarjuna University, Guntur - 522508, A.P,, IN
1 Professor, Department of Pharmaceutical Analysis, Hindu College of Pharmacy, Guntur - 522002, A.P, IN
2 Research Scholar, Acharya Nagarjuna University, Guntur - 522508, A.P,, IN
Source
Research Journal of Pharmacy and Technology, Vol 15, No 3 (2022), Pagination: 1307-1312Abstract
The present research work was aimed to develop a validated UPLC method for simultaneous quantification of Ombitasvir, Paritaprevir and Ritonavir in bulk and pharmaceutical dosage forms. The analytes were successfully separated within 3 mins on waters SB C8 column using pH 4.0 phosphate buffer and acetonitrile in the ratio of 50:50v/v as mobile phase at a flow rate of 0.5ml/min with an injection volume of 0.5μl. The eluted analytes at 0.908, 1.190 and 1.739 min were detected at 250nm by PDA detector. The developed method was validated in compliance with ICH guidelines. The analytes under study obeyed beers law in the concentration range of 3.125 – 18.75μg/ml, 18.75 – 112.5μg/ml and 12.5 - 75μg/ml for Ombitasvir, Paritaprevir and Ritonavir respectively. The percentage recovery of analytes was found to be within 99-101% for the three analytes. The percentage RSD values of precision and robustness were within acceptable limits. The analytes were subjected to various stress conditions such as hydrolysis by acid, alkali and water, oxidation by hydrogen peroxide, exposure to High temperature and UV light, which enables analytes to degrade under these conditions. The proposed method was successful in separating and quantifying the compounds in the presence of degradants. Thus a specific, accurate and robust stability indicating method has been developed for simultaneous quantification of Ombitasvir, Paritaprevir and Ritonavir in their combined dosage form.Keywords
Ombitasvir, Paritaprevir, Ritonavir, UPLC, Stability indicating.References
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